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Plant and Cell Physiology, 1985, Vol. 26, No. 5 805-811
© 1985

Article

Lipid Biosynthesis in Epidermal, Guard and Mesophyll Cell Protoplasts from Leaves of Vicia faba L.

Naoki Sato

Department of Botany, Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo 113, Japan

Protoplasts of epidermal cells, guard cells and mesophyll cells (ECPs, GCPs and MCPs) simultaneously were prepared enzymatically from light-grown leaves of Vicia faba then purified by discontinuous density gradient centrifugation in Percoll. Although the ECPs contained small chlorophyllous plastids, they showed no measurable photosynthetic carbon fixation activity.

Comparable rates of the incorporation of [14C]acetate into the lipid fraction per cell was obtained for ECP, GCP and MCP. Distribution of radioactivity in the lipid classes and fatty acid species was similar in GCP and ECP but differed markedly in MCP. The ability to synthesize C20 and C22 fatty acids was present in GCP and ECP but not in MCP.

(Received January 18, 1985; Accepted April 11, 1985)
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