Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Amino, S.-i.
Right arrow Articles by Komamine, A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Amino, S.-i.
Right arrow Articles by Komamine, A.
Agricola
Right arrow Articles by Amino, S.-i.
Right arrow Articles by Komamine, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1985, Vol. 26, No. 4 745-751
© 1985

Article

Turnover of Cell Wall Polysaccharides during the Cell Cycle in a Synchronous Culture of Catharanthus roseus

Shin-ichi Amino1 and Atsushi Komamine1

Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan

Pulse-chase experiments were done using a synchronous culture of Catharanthus roseus in order to study cell wall turnover during the cell cycle. [14C]Glucose was fed for 1 h to cells 35 and 49 h after the re-start of the cell cycle. Radioactivity was then diluted with a large amount of cold glucose and chased during the early G1 phase after the first cell division, the time at which an increase in the amount of cell walls mainly took place. A pulse-chase with [14C]glucose was also made during the S phase when cell walls had not increased so much.

Radioactivity of the EDTA-soluble (pectin) fraction decreased during the chase in the early G1 phase; whereas, the radioactivities of the other cell wall fractions, as well as extracellular polysaccharide (ECP) increased during the chase, both in the early G1 and in the S phases. The radioactivity of uronic acid in ECP was higher in the early G1 phase than in the S phase. These results indicate that an active turnover of pectin may take place in the early G1 phase after the first cell division.

1 Present address and reprint requests: Biological Institute, Tohoku University, Sendai 980, Japan.

(Received November 5, 1984; Accepted April 2, 1985)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.