Plant and Cell Physiology, 1985, Vol. 26, No. 4 615-625
© 1985
Article |
Interaction of a Brassinosteroid with IAA and GA3 in the Elongation of Cucumber Hypocotyl Sections
Biology Department, International Christian University Osawa, Mitaka, Tokyo 181, Japan
A synthetic brassinosteroid, 22,23(S,S)-homobrassinolide (hBR), was examined for its interaction with IAA and GA3 in the elongation of hypocotyl sections of light-grown cucumber (Cucumis salivus L. cv. Aonagajibai) seedlings. hBR alone was less active than IAA. Its optimal concentration was around 10 µM and the lowest effective concentration between 10 and 100 µM, which is more than 100 times higher than that of brassinolide. hBR was more active in sections from younger seedlings. Its growth-promoting effect was negated or greatly reduced by inhibitors of auxin-induced elongation such as p-chlorophenoxyisobutyric acid and kinetin. hBR acted synergistically with IAA and 2,4-D but not with GA3 showing only an additive effect. Sequential treatment of sections with hBR and then with IAA also resulted in synergistic enhancement of auxininduced elongation, but when the order of treatment was reversed, hBR was inactive. The synergistic effect was obtained with 1 h pretreatment with hBR and could be reduced by subsequent washing with water. There was no sequential interaction between hBR and GA3. The synergistic pretreatment effects of hBR and GA3 were simply additive to each other. A membrane-bound ATPase inhibitor, dicyclohexylcarbodiimide, inhibited the hBR-induced elongation, but did not affect GA3-induced elongation. The findings led to the conclusion that brassinosteroids enhance auxin action and possess growth-promoting activity which is independent of that of gibberellin.
(Received November 9, 1984; Accepted February 18, 1985)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Goda, S. Sawa, T. Asami, S. Fujioka, Y. Shimada, and S. Yoshida Comprehensive Comparison of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis Plant Physiology, April 1, 2004; 134(4): 1555 - 1573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Nemhauser and J. Chory BRing it on: new insights into the mechanism of brassinosteroid action J. Exp. Bot., January 2, 2004; 55(395): 265 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyazawa, N. Nakajima, T. Abe, A. Sakai, S. Fujioka, S. Kawano, T. Kuroiwa, and S. Yoshida Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents J. Exp. Bot., December 1, 2003; 54(393): 2669 - 2678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nakamura, K. Higuchi, H. Goda, M. T. Fujiwara, S. Sawa, T. Koshiba, Y. Shimada, and S. Yoshida Brassinolide Induces IAA5, IAA19, and DR5, a Synthetic Auxin Response Element in Arabidopsis, Implying a Cross Talk Point of Brassinosteroid and Auxin Signaling Plant Physiology, December 1, 2003; 133(4): 1843 - 1853. [Abstract] [Full Text]
|
|
|
|
|
|
C. Mussig, G.-H. Shin, and T. Altmann Brassinosteroids Promote Root Growth in Arabidopsis Plant Physiology, November 1, 2003; 133(3): 1261 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chono, I. Honda, H. Zeniya, K. Yoneyama, D. Saisho, K. Takeda, S. Takatsuto, T. Hoshino, and Y. Watanabe A Semidwarf Phenotype of Barley uzu Results from a Nucleotide Substitution in the Gene Encoding a Putative Brassinosteroid Receptor Plant Physiology, November 1, 2003; 133(3): 1209 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mussig, S. Fischer, and T. Altmann Brassinosteroid-Regulated Gene Expression Plant Physiology, July 1, 2002; 129(3): 1241 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Kim, S. C. Chang, E. J. Lee, W.-S. Chung, Y.-S. Kim, S. Hwang, and J. S. Lee Involvement of Brassinosteroids in the Gravitropic Response of Primary Root of Maize Plant Physiology, July 1, 2000; 123(3): 997 - 1004. [Abstract] [Full Text]
|
|