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Plant and Cell Physiology, 1985, Vol. 26, No. 3 571-578
© 1985

Article

Phosphate Compounds of Scenedesmus C-2A' in Darkness or Blue Light as Measured by 31P NMR

Günter Ruyters1, Tamiko Oh-hama2 and Wolfgang Kowallik1

1 Faculty of Biology, University of Bielefeld 4800 Bielefeld 1, W-Germany
2 Institute of Applied Microbiology, University of Tokyo Tokyo 113, Japan

Phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of intact cells of Scenedesmus mutant C-2A' and of their perchloric acid extracts are presented. Sugarphosphates, including glucose-6-phosphate and fructose-1,6-bisphosphate, orthophosphate, nucleotide di- and triphosphates, NAD(P), UDPG and—in the case of intact cells—also polyphosphates were identified. Blue light, which is known to stimulate the carbohydrate breakdown of green algae, leads to a transient drop in Pi, a pronounced decrease in the ATP/ADP ratio, and an increase in sugarphosphates, giving rise to the idea that the enhancement of phosphorolytic starch breakdown is a primary response to blue light. Addition of glucose to Scenedesmus mutant cells leads to comparable changes (besides an additionally enhanced glucose-6-phosphate level), which thus support the view that blue light stimulates dark-type respiration. Altogether the results demonstrate the applicability of 31P NMR spectroscopy to the study of the metabolism of green algae.

(Received December 7, 1984; Accepted February 12, 1985)
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