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Plant and Cell Physiology, 1985, Vol. 26, No. 3 537-542
© 1985

Article

Effects of CaCl2-washing on EPR Signals of Cytochrome b559 in Photosystem II Preparation from Spinach Chloroplasts

Katsumi Matsuura1 and Shigeru Itoh

National Institute for Basic Biology Okazaki, Aichi 444, Japan

Washing of PS II preparation by 1 M CaCl2 inactivates oxygen evolution without loss of bound manganese [Ono and Inoue (1983) FEBS Lett. 164: 255]. Most of the high-potential Cyt b550, which amounts to about a half of the total Cyt b559 in untreated preparation, was converted to its low-potential form by CaCl2-washing. The effect was similar to that of Tris-washing. The peak position of the gs band of the EPR spectrum of the CaCl2-washed preparation (g=2.95) was the same as that of the low potential form of untreated preparation but was slightly different from that of the Tris-washed or heat-treated preparation (g=2.98).

1 Present address: Department of Biology, Faculty of Science, Tokyo Metropolitan University, Fukazawa 2-1-1, Setagaya, Tokyo 158, Japan.

(Received November 14, 1984; Accepted January 30, 1985)
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