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Plant and Cell Physiology, 1985, Vol. 26, No. 3 515-524
© 1985

Article

Development of Lipid Synthesis from CO2 in Avena Leaves during Greening of Etiolated Seedlings

Shin-ichiro Watanabe and Mitsuhiro Yamada

Department of Biology, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan

The development of the lipid synthesizing system in Avena leaf sections was examined in connection with carbon fixation during the greening of etiolated seedlings under light. During the initial 2 h illumination there was a low level of CO2 fixation by PEP carboxylation, but its products, malate and citrate, did not serve as a carbon source for lipid synthesis, although lipid synthesis from acetate had already been established. With the initiation of Calvin cycle activity after the initial 2 h illumination, lipid synthesis began, with CO2 fixed by RuBP carboxylation serving exclusively as the carbon source. Fatty acid synthesis in the leaves during the initial 3 h illumination, unlike the fatty acid synthesis thereafter, was insensitive to thiolactomycin, an inhibitor of type II fatty acid synthetase contained in the plastids, and was not dependent on light, in contrast to light-dependent activity in greened leaves.

The distribution of 14C incorporated into lipid molecules from NaH14CO3 showed an equal ratio of 14C in fatty acid, glycerol and choline moieties of labeled phosphatidylcholine, but a denser radioactivity in the galactose moiety than in the residual moiety of mono- and di-galactosyldiacylglycerols. This suggests a regulated supply of glycerol, choline and fatty acid moieties for phosphatidylcholine synthesis, and an excess supply of galactose to diacylglycerol moiety for galactosyldiacylglycerol synthesis in Avena leaves.

(Received October 31, 1984; Accepted January 25, 1985)
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