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Plant and Cell Physiology, 1985, Vol. 26, No. 2 309-315
© 1985

Article

Isolation and Characterization of Photosynthetically Active Cells from Submersed and Floating Leaves of the Aquatic Macrophyte Potamogeton nodosus Poir

Frederick J. Ryan

USDA-ARS Aquatic Weed Control Research Laboratofy, Botany Department, University of California Davis, CA 95616, U.S.A.

Photosynthetically active cells were isolated by enzymic digestion of floating and submersed leaves of the heterophyllous aquatic macrophyte Potamogeton nodosus Poir. The yields of cells isolated from floating leaves represented approximately 25% of the leaf protein or chlorophyll, while cell yields from submersed leaves were only 3%. Photosynthetic activity was maximal in cells isolated from submersed leaves 10 to 14 days after germination of the winterbuds. Floating leaves were induced by treatment of the plants with abscisic acid. Cells from induced floating leaves showed maximum photo synthetic rates between 9 and 21 days posttreatment. Phosphoglycerate, fructose 1,6-bisphosphate, sulfate and phosphate were without significant effect on photosynthesis in either cell type indicating that the cells were substantially intact. Half-saturation of photosynthesis for bicarbonate was at 0.6 mM (pH 7.6) for cells from both leaf types, and the maximum rate was greater for cells from floating leaves. The light intensity for half-saturation of photosynthesis was approximately 95 µE m–2s–1 for cells from both leaf types, and the maximum rate was greater for cells from floating leaves.

(Received September 19, 1984; Accepted December 6, 1984)
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