Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Osafune, T.
Right arrow Articles by Hase, E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Osafune, T.
Right arrow Articles by Hase, E.
Agricola
Right arrow Articles by Osafune, T.
Right arrow Articles by Hase, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1985, Vol. 26, No. 2 263-270
© 1985

Article

Interactions between the Nucleus and Cytoplasmic Organelles during the Cell Cycle of Euglena gracilis in Synchronized Cultures

II. Associations between the Nucleus and Chloroplasts at an Early Stage of the Cell Cycle under Photoautotrophic Conditions1

Tetsuaki Osafune2, Tomoko Ehara2, Shuji Sumida2 and Eiji Hase3

2 Department of Microbiology, Tokyo Medical College 6-1-1, Shinjuku, Tokyo 160, Japan
3 Laboratory of Chemistry, Faculty of Medicine, Teilcyo University Otsuka 359, Tokyo 192-03, Japan

Chloroplast-nucleus interactions were examined in cells of Euglena gracilis Z synchronized under photoautotrophic conditions. The chloroplasts were localized near the cell periphery. At an early stage of the cell cycle, however, some chloroplasts were transiently located in the inner space close to the nucleus. Electron microscopy using serial cell sections revealed that the chloroplast formed protrusions at several sites, which became associated with the nucleus. The outer membrane of the chloroplast envelope was in contact, or at least continuous in part, with the outer membrane of the nuclear envelope at the sites of association, and dense material was present in the chloroplast membrane. A chromosome was close to each site of the association between these two organelles. Most of the chloroplasts including those in association with the nucleus were connected by fine bridges. The 4',6-diamidino-2-phenylindole-stained nucleoids in the chloroplast associated with the nucleus appeared to have a thread-like shape. There was another type of chloroplast-nucleus connection, in which an intervening membranous body was in contact with the outer part of the nuclear envelope on one side and with the chloroplast envelope on the other side.

1 This work was reported at the 48th Annual Meeting of the Botanical Society of Japan, Kyoto, October, 1983.

(Received June 5, 1984; Accepted November 20, 1984)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.