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Plant and Cell Physiology, 1985, Vol. 26, No. 2 237-244
© 1985

Article

Ethylene-Enhanced Transport of Uranine, a Fluorescent Dye, in Rice-Seedling Explants in Relation to Ethylene-Stimulated Coleoptile Growth

Kimiharu Ishizawa and Yohji Esashi

Department of Biological Science, Tohoku University Kawauchi, Sendai 980, Japan

Ethylene enhanced the transport of uranine, a fluorescent dye, through the coleoptiles of rice (Oryza sativa L. cv. Sasanishiki) seedlings explants after a 2-h lag period. The uranine transport increased with increasing concentration of ethylene, reaching a maximum at 1 µ1.liter–1 ethylene. It decreased when some metabolic inhibitors, 2,4-dinitrophenol, carbonyl cyanide 3-chlorophenylhydrazone and N-ethylmaleimide, were applied to the scutella. Mechanical rupture or heating at the vascular-bundle sides of the coleoptiles also interfered with the ethylene-enhanced transport of uranine. The wounding and removal of the scutella similarly blocked uranine transport. Moreover, strong fluorescence of uranine was observed on the phloem regions of two vascular bundles of a coleoptile under a fluorescence microscope. It is suggested that the ethylene-enhanced transport of uranine in the coleoptile of rice-seedling explants occurs through the phloem. On the other hand, the removal of the endosperm or the rupture of the vascular bundles inhibited the ethylene-stimulated elongation of the rice coleoptiles. Moreover, ethylene increased dry weight of the shoots. These results suggest that the ethylene-stimulated elongation of rice coleoptiles was supported partly by the ethylene-enhanced translocation of materials from the endosperms to the coleoptiles.

(Received September 6, 1984; Accepted November 15, 1984)
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