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Plant and Cell Physiology, 1985, Vol. 26, No. 2 221-228
© 1985

Article

Promotion of Rooting in Azukia Cuttings by Possible Glycoproteins Extracted from Lentinus edodes Culture

Mieko Mitsuhashi-Kato1, Hiroaki Maeda2 and Tadashi Fujii3

1 Junior College of Toyo University Hakusan, Bunkyo-ku, Tokyo 112, Japan
2 Noda Shokukin Kogyo Co. Ltd. Shimizu, Noda City, Chiba 278, Japan
3 Institute of Biological Sciences, University of Tsukuba Sakura-mura, Ibaraki 305, Japan

Macromolecules purified from Lentinus edodes mycelia culture enhanced adventitious root formation in Azukia epicotyl cuttings. Partial purification by sequential column chromatographies gave material composed of 71% polysaccharides and 29% proteins. The sugar moiety consisted of mainly xylose, glucose and arabinose, the sum of their contents being more than 76% of the total carbohydrates. The protein moiety consisted of mainly glycine and serine, which accounted for more than 40% of the total amino acid residues.

(Received June 9, 1984; Accepted November 12, 1984)
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