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Plant and Cell Physiology, 1985, Vol. 26, No. 1 63-75
© 1985

Article

Palisade Tissue Chloroplasts and Spongy Tissue Chloroplasts in Spinach: Biochemical and Ultrastructural Differences

Ichiro Terashima1 and Yorinao Inoue2

1 Laboratory of Ecology, Department of Botany, Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo 113, Japan
2 Solar Energy Research Group, Riken Institute of Physical and Chemical Research Hirosawa, Wako-shi, Saitama 351-01, Japan

Palisade tissue chloroplasts (P-Chlts) and spongy tissue chloroplasts (S-Chlts) were separately isolated from spinach leaves, and their photosynthetic properties were compared. The following results were obtained:

(1) At saturating light, the activities of overall electron transport and CO2 fixation in P-Chlts were respectively 1.6–2.0 and 2.5–3.0 times higher than those in S-Chlts on a Chl basis.

(2) The contents of PS I and PS II reaction centers (P700 and 47 kDa polypeptide, respectively) were slightly higher in P-Chlts than in S-Chlts, while the contents of plastoquinone, Cyt f, plastocyanin, ferredoxin, ferredoxin-NADP+ reductase, coupling factor and ribulose-bisphosphate carboxylase were 1.6–2.2 times higher in P-Chlts than in S-Chlts on a Chl basis.

(3) Electron microscopic examination of chloroplast ultrastructure showed that S-Chlts have highly stacked grana accompanied by higher proportion of appressed thylakoids relative to non-appressed thylakoids, while P-Chlts have poorly stacked grana. The volume ratio of thylakoids to stroma was higher in S-Chlts than in P-Chlts.

These results indicate that mesophyll chloroplasts adapt to the light environment within a leaf in a similar way that the sun and shade plant chloroplasts adapt to the light environment within a canopy.

(Received July 19, 1984; Accepted October 13, 1984)
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