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Plant and Cell Physiology, 1984, Vol. 25, No. 7 1303-1306
© 1984

Short Communication

Changes in Endogenous Gibberellin Contents during Growth of Cultured Tobacco Cells

Keun-Hyung Park1,3, Sachiko Fujisawa2, Akira Sakurai1,4, Isomaro Yamaguchi2 and Nobutaka Takahashi1,2

1The Institute of Physical and Chemical Research Wako-shi, Saitama 351, Japan
2Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan

4 To whom all correspondence should be addressed.

Changes in the contents of endogenous gibberellins (GAs) were examined in three kinds of cultured tobacco cells; a crown gall cell and two cultured cells derived from normal tissue of Nicotiana tabacum. The relative amounts of the GAs were analyzed by systematic chromatographic purifications followed by GC-SIM. In all the cell lines examined, the content of GAj was the highest during the logarithmic phase of growth, indicating that GA1 has a physiological role in the growth of dedifferentiated cells.

3 Present address: College of Agriculture, Chonnam National University, Kwangju 500, Korea.

(Received April 11, 1984; Accepted July 10, 1984)
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