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Plant and Cell Physiology, 1984, Vol. 25, No. 7 1255-1264
© 1984

Article

Separation and Properties of the Inner and Outer Membranes of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1

Hidetoshi Okuyama, Hiroko Itoh1, Noriyuki Fukunaga and Shoji Sasaki

Department of Biology, Faculty of Science, Hokkaido University Sapporo 060, Japan
1Sorachi Branch Office of the Hokkaido Government Present address: Iwamizawa 068, Japan

The inner and outer membranes from a marine psychrophilic bacterium, Vibrio sp. ABE-1, were separated and purified by desalting, lysozyme treatment, and ultracentrifugation. Enzyme assay, electrophoresis, and chemical analysis showed that they had been highly purified. The isolated outer membrane had no 3-deoxy-D-mannooctulosonate (KDO) and chemical analysis revealed that it contained about three times as much total lipid as the inner membrane. Consequently, the density of the inner membrane was higher than that of the outer membrane (1.19–1.23 gµcm3 for IM; 1.16–1.20 g–cm3 for OM). The absolute majority of the lipids in these membranes were phospholipids, more than half of which was identified as phosphatidylethanolamine. Virtually all cardiolipin was found exclusively in the inner membrane, whereas an unidentified phospholipid containing hexosamine was only in the outer membrane. The ratios of unsaturated to total fatty acids in the inner and outer membranes were 76.0% and 69.6%, respectively.

(Received February 23, 1984; Accepted August 8, 1984)
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