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Plant and Cell Physiology, 1984, Vol. 25, No. 7 1247-1254
© 1984

Article

Paraquat Resistant Tobacco Calluses with Enhanced Superoxide Dismutase Activity

Iwao Furusawa1, Kunisuke Tanaka2, Porntip Thanutong1, Atsuo Mizuguchi1, Motoki Yazaki2 and Kozi Asada3

1Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto University Kyoto 606, Japan
2Department of Biochemistry, College of Agriculture, Kyoto Prefectural University Kyoto 606, Japan
3The Research Institute for Food Science, Kyoto University Uji, Kyoto 611, Japan

3K. Asada, The Research Institute for Food Science, Kyoto University, Uji, Kyoto 611, Japan.

Seven paraquat resistant calluses of tobacco (Nicotiana tabacum L. cv. Samsun) were obtained by three successive screenings of protoplast-derived calluses on a paraquat containing medium. Superoxide dismutase (SOD) activity of the resistant calluses was 14- to 159-fold that of the leaf cells on protein basis. Paraquat-resistant calluses, however, showed little increase in catalase and peroxidase activities. More than 90% of SOD activity in the resistant calluses was inhibited by KCN, as was the SOD activity in leaves, indicating that the major SOD in the callus appears to be the Cu, Zn containing enzyme. The callus cells, however, expressed the immunologically distinguished SOD isozyme from the enzyme in the leaves.

(Received April 23, 1984; Accepted August 6, 1984)
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