Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Satoh, H.
Right arrow Articles by Miyaji, K.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Satoh, H.
Right arrow Articles by Miyaji, K.
Agricola
Right arrow Articles by Satoh, H.
Right arrow Articles by Miyaji, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1984, Vol. 25, No. 7 1205-1214
© 1984

Article

Purification and Further Characterization of Pyrenoid Proteins and Ribulose-1,5-bisphosphate Carboxylase-Oxygenase from the Green Alga Bryopsis maxima

Hiroyuki Satoh, Mitsumasa Okada, Katsumi Nakayama and Kazuyuki Miyaji

Department of Biology, Faculty of Science, Toho University 2-2-1 Miyama, Funabashi, Chiba 274, Japan

Pyrenoid proteins and ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) in the green alga Bryopsis maxima were purified to high degrees and their peptide compositions were studied by SDS-polyacrylamide gel electrophoresis. RuBisCO had a large subunit of 50 kDa and a small one of 16 kDa. The apparent molecular weight of the purified RuBisCO was estimated as 460 kDa by gel filtration. Pyrenoid proteins had two major polypeptides: 52 kDa and 17 kDa. The peptide map of the 52 kDa pyrenoid polypeptide coincided well with that of the large subunit of RuBisCO, strongly suggesting that the major component of the pyrenoid of this alga was RuBisCO. We attempted to survey the distribution of RuBisCO in the chloroplasts. The results suggested that much of the RuBisCO of Bryopsis maxima was localized in the pyrenoid. The pyrenoid also contained more than 10 minor polypeptides not found in the RuBisCO fraction. The minor polypeptides comprised about 15% of the total pyrenoid protein and differed from the polypeptides of the thylakoid membranes and from those found in the starch grains surrounding the pyrenoid.

(Received February 3, 1984; Accepted July 21, 1984)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.