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Plant and Cell Physiology, 1984, Vol. 25, No. 7 1107-1113
© 1984

Article

Effect of Intracellular ATP Levels on the Light-Induced H+ Efflux from Intact Cells of Cyanidium caldarium1

Isao Enami and Mariko Kura-Hotta2

Department of Biology, Faculty of Science, Science University of Tokyo Kagurazaka, Shinjuku-ku, Tokyo 162, Japan
2Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo Present address: Komaba, Meguro-ku, Tokyo 153, Japan

The light-induced H+ efflux observed at acidic pH in Cyanidium cells was shown to be an active H+ transport depending on the intracellular ATP produced by cyclic photo-phosphorylation. Triton X-100 was found to act as an effective uncoupler in intact Cyanidium cells without collapsing the pH gradient across the plasma membrane. Triton X-100 at 0.015% significantly reduced the intracellular ATP levels, stimulated the p-BQ, Hill reaction and completely inhibited the light-induced H+ efflux. Inhibition of the H+ efflux by Triton X-100 correlated well with the depression of the apparent rale of light-induced ATP synthesis as well as the decrease in the intracellular ATP level in light.

The light-induced H+ efflux was completely inhibited by diethylstilbestrol, a specific inhibitor of plasma membrane ATPase, without any changes in the intracellular ATP level, thereby suggesting the participation of the plasma membrane ATPase in the light-induced H+ efflux.

1The data in this paper are included in the Ph. D. dissertation submitted by M. Kura-Hotta to Tokyo Metropolitan University.

(Received February 3, 1984; Accepted June 14, 1984)
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