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Plant and Cell Physiology, 1984, Vol. 25, No. 6 947-953
© 1984

Article

Digitoxin Biosynthesis in Isolated Mesophyll Cells and Cultured Cells of Digitalis

Manabu Hagimori, Takashi Matsumoto and Yoichi Mikami

Central Research Institute, The Japan Tobacco & Salt Public Corporation 6-2 Umegaoka, Midori-ku, Yokohama 227, Japan

In the laminae of Digitalis, most of the digitoxin present is found in the mesophyll. A new method for determining the amount of digitoxin biosynthesis using a digitoxin antibody was devised to estimate this activity in isolated mesophyll cells and cultured cells. Isolated mesophyll cells showed significant activity, which suggests that the site of biosynthesis and the accumulation of cardenolides in a lamina of Digitalis is mainly in the mesophyll cells. Of five liquid cultures of D. purpurea; green shoot-forming cultures, white shoot-forming cultures, root-forming cultures, undifferentiated green cells and undifferentiated white cells, the green shoot-forming cultures had the highest activity. The white shoot-forming cultures had about one-third the activity of the green shoot-forming cultures, and the other three cultures had very low activity. No stimulatory effect of light was found during the 48-h incubation.

(Received January 19, 1984; Accepted June 8, 1984)
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