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Plant and Cell Physiology, 1984, Vol. 25, No. 6 883-889
© 1984

Article

Occurrence of Cu,Zn-Superoxide Dismutase in the Intrathylakoid Space of Spinach Chloroplasts

Takahiko Hayakawa, Sumio Kanematsu and Kozi Asada

The Research Institute for Food Science, Kyoto University Uji, Kyoto 611, Japan

Thylakoids obtained from intact spinach chloroplasts showed no superoxide dismutase (SOD) activity, but Cu,Zn- and Mn-SOD activities were detected in the presence of Triton X-100. Thylakoid membranes and the lumen fraction were separated by centrifugation after treatment of the thylakoids with a Yeda pressure cell. Cu,Zn-SOD was found in the lumen fraction. Mn-SOD was detected in the thylakoid fraction only after addition of 1% Triton X-100. Antibody against spinach Cu,Zn-SOD did not interact with the latent Cu,Zn-SOD in the thylakoids unless Triton was added. These results indicate that Cu,Zn-SOD occurs in the lumen in addition to the stroma of spinach chloroplasts, and Mn-SOD binds to the thylakoid membranes.

(Received February 29, 1984; Accepted May 28, 1984)
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