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Plant and Cell Physiology, 1984, Vol. 25, No. 6 867-873
© 1984

Article

Two Different Soluble Cytoplasmic Auxin-Binding Sites in Etiolated Pea Epicotyls

Hans-Jörg Jacobsen

Institut für Genetik der Universität Bonn Kirschallee 1, D-5300 Bonn-1, FRG

Etiolated pea epicotyls from 7–9 day-old seedlings exhibit the existence of only one class of soluble auxin binding sites, while later stages (10–12 days) obviously have two: A second class of sites appears in a protein fraction which by means of chromatofocusing can be separated from the first. The pI-ranges are 5.0–6.0 for the first and 6.0–6.8 for the second class of sites, respectively. Double reciprocal plots show that there is no competition between the sites, which obviously have different KD (0.55×10-7 M vs. 2.24×10-7 M). Evidence is presented that soluble auxin binding is not likely to be due to binding to NAA-conjugating enzymes, since no conjugation products can be found in supernatants after binding assays.

(Received February 27, 1984; Accepted May 22, 1984)
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