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Plant and Cell Physiology, 1984, Vol. 25, No. 6 1099-1102
© 1984

Short Communication

Photoautotrophic Culture of Undifferentiated Cells and Shoot-Forming Cultures of Digitalis purpurea L.

Manabu Hagimori, Takashi Matsumoto and Yoichi Mikami

Central Research Institute, The Japan Tobacco & Salt Public Corporation 6–2 Umegaoka, Midori-ku, Yokohama 227, Japan

Undifferentiated cells and shoot-forming cultures of Digitalis purpurea L. were grown photoautotrophically under 1% CO2. During 3 weeks of culture, the undifferentiated cells multiplied 3-fold and the shoot-forming cultures 2-fold on a fresh weight basis. The chlorophyll content, ribulose 1,5-bisphosphate carboxylase activity, Hill reaction activity of the isolated chloroplasts and photosynthetic O2 evolution of the photoautotrophically grown cultures were somewhat higher than the values of the corresponding photomixotrophic cultures. The digitoxin contents, however, were not improved by photoautotrophic culture.

(Received November 9, 1983; Accepted June 11, 1984)
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