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Plant and Cell Physiology, 1984, Vol. 25, No. 6 1069-1071
© 1984

Short Communication

Cell-Free Synthesis of a Putative Precursor of Polygalacturonase in Tomato Fruits

Takahide Sato, Sakiku Kusaba, Hiroki Nakagawa and Nagao Ogura

Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University Matsudo, Chiba 271, Japan

By using a monospecific anti-polygalacturonase-2 antibody, a 54K-dalton polypeptide was detected in in vitro translation products by a wheat germ cell-free translation system programmed with polyadenylated RNA from ripe tomato pericarp tissue. This putative precursor of polygalacturonase was about 9K daltons larger in molecular weight than polygalacturonase-2.

(Received December 12, 1983; Accepted May 17, 1984)
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