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Plant and Cell Physiology, 1984, Vol. 25, No. 6 1017-1025
© 1984

Article

Kinetics of Endogenous Cytokinin, IAA and ABA Levels in Relation to the Growth and Morphology of Tobacco Crown Gall Tissue

Henri Van Onckelen1,4, Patrick Rudelsheim1,6, Robert Hermans1, Stefaan Horemans1, Eric Messens2, Jean-Pierre Hernalsteens3,5, Marc van Montagu2,3 and Jan De Greef1

1Department of Biology UIA, B-2610 Wilrijk, Belgium
2Laboratorium voor Genetica, RUG B-9000 Gent, Belgium
3Laboratorium voor Genetische Virologie, VUB B-1640 St. Genesius-Rode, Belgium

The kinetics of endogenous cytokinin, IAA and ABA levels during the growth cycle of a wild-type tobacco crown gall (W38-B6S3) were compared with that of a shoot-inducing (Shi-) mutant. In both tumor types, high IAA and cytokinin (essentially ribosyl-trans-zeatin and its corresponding glucoside) levels were built up by the end of the linear growth phase and maintained during the greater part of the exponential growth period. The stationary phase was preceded by a very drastic decrease in the endogenous level of both hormones.

Quantitatively, the wild-type tumour showed a higher IAA level and a reduced cytokinin level compared with the Shi- mutant. No significantly different endogenous ABA pattern was observed. The reduced cytokinin level might correspond to the ratio of transformed/untransformed cells in the wild-type tumour whereas the reduced IAA level in the Shi- mutant may be correlated with the deletion of gene 2 in the T-DNA of the pGV 2206 Ti plasmid.

The elevated cytokinin/IAA ratio induced shooting mainly of the untransformed cells in the Shi- mutant tissue whereas in the wild-type, the shoot suppression was compatible with the reduced cytokinin/IAA ratio.

4Senior Research Associate Nationaal Fonds Wetenschappelijk Onderzoek (N.F.W.O.).

5Research Associate N.F.W.O.

6Recipient of an Instituut voor Wetenschappelijk Onderzoek in Nijverheid en Landbouw grant.

(Received February 23, 1984; Accepted June 19, 1984)
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