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Plant and Cell Physiology, 1984, Vol. 25, No. 5 821-830
© 1984

Article

Protein Synthesis in Isolated Plastids during Chloroplast Development in Wheat

Junichi Obokata1

Department of Botany, Faculty of Science, Hokkaido University Sapporo 060, Japan
1Research Reactor Institute, Kyoto University Present address: Kumatori-cho Sennan-gun, Osaka 590-04, Japan

Light-driven protein synthesis in isolated plastids was studied during the greening of etiolated wheat (Triticum aestivum L.) seedlings. The process was divided into five phases (I to V) according to the recovery of plastids from the leaf tissue. The activity was not detected in the etioplasts, but rapidly increased to the maximum level in phase I and remained at this level through phase II. During the transition from phase II to III, the activity rapidly decreased to one-third and then continued to decrease slowly. The plastid polypeptides synthesized during the greening were analyzed by SDS-polyacrylamide gel electrophoresis. In phase I, membrane polypeptides having molecular weights of about 21k were synthesized, while 23 k membrane polypeptide was synthesized in phases III, IV and V. Synthesis of soluble polypeptides of 50–60 k and membrane polypeptides of 15 k and 30–35 k was active in phases I and II, but decreased between phases II and III.

(Received October 31, 1983; Accepted May 14, 1984)
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