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Plant and Cell Physiology, 1984, Vol. 25, No. 5 775-783
© 1984

Article

Carbonic Anhydrase from the Blue-Green Alga (Cyanobacterium) Anabaena variabilis

Yuriko Yagawa1, Yoshihiro Shiraiwa1 and Shigetoh Miyachi2,2

1Department of Biology, Faculty of Science, Niigata University Niigata 950-21, Japan
2Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

2To whom requests for reprints should be addressed.

Carbonic anhydrase (CA) activity was detected in homogenates from Anabaena variabilis ATCC 29413, M-2 and M-3, but not in the suspension of the intact cells. Activity was higher in cells grown in ordinary air (low-CO2 cells) than in those grown in air enriched with 2–4% CO2 (high-CO2 cells). Fractionation by centrifugation indicated that the CA from A. variabilis ATCC 29413 is soluble, whereas both soluble and insoluble forms exist in A. variabilis M-2 and M-3. The addition of dithiothreitol and Mg2 $ greatly decreased the CA activity of A. variabilis ATCC 29413.

The specific activity of the CA from A. variabilis ATCC 29413 was increased ca. 200 times by purification with ammonium sulfate, DEAE-Sephadex A-50 and Sephadex G-100. Major and minor CA peaks in Sephadex G-100 chromatography showed respective molecular weights of 48,000 and 25,000. The molecular weight of the CA determined by polyacrylamide disc gel electrophoresis was 42,000±5,000. The activity of CA was inhibited by ethoxyzolamide (I50=2.8×10-9 M), acetazolamide (I50=2.5×10-7 M) and sulfanilamide (I50=2.9×10-6 M).

(Received January 5, 1984; Accepted April 26, 1984)
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