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Plant and Cell Physiology, 1984, Vol. 25, No. 5 763-768
© 1984

Article

Isolation and Properties of Intact Chromoplasts from Tomato Fruits

Norio Iwatsuki, Ryuichi Moriyama and Tadashi Asahi

Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University Chikusa, Nagoya 464, Japan

Intact chromoplasts were isolated from tomato fruits at different ripening stages by Percoll density gradient centrifugation. The isolated chromoplast fractions were contaminated very little by other organelles, although the fraction from fully ripened fruits contained some mitochondria and microbodies. As the transformation of chloroplasts to chromoplasts proceeded, the density of the plastids decreased from 1.096 to 1.075 g-cm-3 and the decrease was related to a decrease in chlorophyll and an increase in lycopene in the plastids.

(Received December 21, 1983; Accepted April 20, 1984)
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