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Plant and Cell Physiology, 1984, Vol. 25, No. 5 749-756
© 1984

Article

Effects of Ammonia on Respiration, Adenylate Levels, Amino Acid Synthesis and CO2 Fixation in Cells of Chlorella vulgaris 11h in Darkness

Masayuki Ohmori1,3, Shizuko Miyachi2, Kei-ichiro Okabe3,4 and Shigetoh Miyachi2,3

1Ocean Research Institute, University of Tokyo Nakano-ku, Tokyo 164, Japan
2Radioisotope Centre, University of Tokyo Bunkyo-ku, Tokyo 113, Japan
3Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

The effects of NH4Cl on respiration, adenylate and free amino acid levels as well as dark CO2 fixation were investigated using nitrogen-starved Chlorella vulgaris 11h cells with or without addition of methionine sulfoximine (MSX), an inhibitor of glutamine synthetase. Upon addition of NH4Cl (1 mM) to the cells not treated with MSX, respiration was stimulated and the level of ATP dropped rapidly, while the levels of ADP and AMP increased. NH4Cl also stimulated amino acid synthesis, especially of glutamine, and markedly enhanced dark CO2 fixation. Addition of NH4Cl to MSX-treated cells stimulated respiration and lowered the level of ATP, but did not enhance glutamine synthesis and only slightly stimulated dark CO2 fixation.

4On leave from Institute of Medical Science, Advance R & D Co. Minami-Hashimoto, Sagamihara, Kanagawa-ken 220, Japan

(Received January 28, 1984; Accepted April 19, 1984)
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