Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ealing, P. M.
Right arrow Articles by Scott, K. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ealing, P. M.
Right arrow Articles by Scott, K. J.
Agricola
Right arrow Articles by Ealing, P. M.
Right arrow Articles by Scott, K. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1984, Vol. 25, No. 5 739-747
© 1984

Article

Powdery Mildew Infection Alters in Vitro Transcription Patterns in Wheat Leaf Nuclei

Paul M. Ealing, Arun K. Chakravorty and K. J. Scott

Department of Biochemistry, University of Queensland St. Lucia, Queensland 4067, Australia

We have isolated transcriptionally active nuclei from a cultivar of wheat (Triticum aestivum L., var Zenith) which is susceptible to race 1 of the powdery mildew fungus (Erysiphe graminis f. sp. tritici). There are two distinct peaks of in vitro RNA synthesis at (NH4)2SC4 concentrations of 20 mM and 240 mM respectively. The effects of {alpha}-amanitin on RNA synthesis by the isolated nuclei have suggested that the peak at 20 mM is due mainly to the activity of wheat leaf RNA polymerases I and III whilst that at 240 mM is due mostly to RNA polymerase II. There is a significant increase in nuclear RNA synthesis at both (NH4)2SO4 concentrations beginning at two days after inoculation of the wheat leaves with the powdery mildew fungus. Analytical gel electrophoresis of the RNA radiolabelled in vitro at 240 mM (NH4)2SO4 has revealed that the nuclei from inoculated leaves preferentially synthesize some high molecular weight RNA presumably representing mRNA precursor molecules. These results suggest quantitative and qualitative changes in the nuclear transcription patterns of wheat leaves during the early stages of disease development.

(Received December 3, 1983; Accepted April 16, 1984)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.