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Plant and Cell Physiology, 1984, Vol. 25, No. 1 63-67
© 1984

Article

Presence of Organelle-Associated Serine Enzymes in Spinach Leaves

Shinobu Satoh and Tadashi Fujii

Institute of Biological Sciences, University of Tsukuba Sakura-mura, Ibaraki 305, Japan

Particulate fractions prepared from spinach leaves by differential centrifugation were analyzed for proteins capable of binding di-isopropyl phosphorofluoridate (DFP); [3H]-labeled DFP and SDS-polyacrylamide gel electrophoresis were used. The chloroplast-rich fraction contained one kind of DFP-binding protein, whose binding was inhibited by phenylmethylsulfonyl fluoride (PMSF). The mitochondrion-rich fraction contained another DFP-binding protein, whose binding was sensitive to PMSF and L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK). The microsome-rich fraction contained three PMSF-sensitive DFP-binding proteins; one was sensitive to both Np-tosyl-L-lysine chloromethyl ketone (TLCK) and TPCK, one was sensitive to TLCK and one to TPCK. These DFP-binding proteins are believed to be serine proteases.

(Received April 30, 1983; Accepted October 17, 1983)
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