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Plant and Cell Physiology, 1984, Vol. 25, No. 1 27-38
© 1984

Article

Changes in Endogenous Amino Acid Compositions during Somatic Embryogenesis in Daucus carota L.

Hiroshi Kamada and Hiroshi Harada

Institute of Biological Sciences, University of Tsukuba Sakura-mura, Ibaraki-ken 305, Japan

Changes in amino acid compositions of carrot cells cultured in three different media were examined in relation to somatic embryogenesis. The total amount of amino acids in the 80% ethanol-insoluble fractions increased rapidly during cell proliferation and globular embryo formation. The individual proportions of the amino acids in these fractions remained fairly constant irrespective of the development of the somatic embryos. The total amount of amino acids in the 80% ethanol-soluble fractions varied depending on the medium used.

Continuously applied {alpha}-alanine did not accumulated in the cells, which suggests that it was transformed to other amino acids during culture. A large amount of {gamma} aminobutyric acid was detected throughout the culture period. Glutamic acid and glutamine accumulated in the carrot cells during embryo formation and maturation. Changes in the compositions and amounts of the endogenous amino acids present during embryo formation are discussed.

(Received April 28, 1983; Accepted October 5, 1983)
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