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Plant and Cell Physiology, 1982, Vol. 23, No. 8 1443-1452
© 1982

Article

Synthesis of Arabinose-Containing Cell Wall Precursors in Suspension-Cultured Tobacco Cells II. Partial Purification and Some Physical Characterization of an Intracellular Precursor of Cell Wall Glycoproteins

Shinji Kawasaki

Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan

Arabinose-containing macromolecules accumulate in the Golgi apparatus prior to their secretion into the cell wall. These intracellular macromolecules of suspension-cultured tobacco cells were labelled with 14C-arabinose, extracted and partially characterized. The major component is a glycoprotein, in which arabinose is linked to hydroxyproline residues as oligosaccharides, among them mainly as disaccharides. A pulse-chase experiment showed the major glycoprotein to be a precursor of cell-wall materials. The glycoprotein has a density of 1.65 g/cm3, indicating a high content of carbohydrate ({gtrsim}90%), of which arabinose, galactose and uronates are the major components. The glycoprotein is highly acidic (pIlsim1.3), probably due to the presence of uronate. The large stokes' radius, equivalent to a 5 × 105-dalton protein, and a small S value (6.5 S) indicate a swollen structure for the molecule. These data indicate a close similarity of the glycoprotein to an extracellular arabinogalactan protein secreted into the culture medium.

Present address: Institute for Plant Virus Research, Tsukuba Science City, Yatabe, Ibaraki 305, Japan.

(Received April 12, 1982; Accepted October 20, 1982)
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