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Plant and Cell Physiology, 1982, Vol. 23, No. 8 1435-1441
© 1982

Article

Effects of Calmodulin Antagonists on the Mitochondrial and Microsomal Ca2$ Uptake in Apple Fruit1

Masashi Fukumoto and Koushiro Nagai

Morioka Branch, Fruit Tree Research Station, Ministry of Agriculture, Forestry and Fisheries Shimokuriyagawa, Morioka 020-01, Japan

In apple fruit, active ATP-dependent microsomal Ca2$ uptake and respiration-dependent mitochondrial Ca2$ uptake were observed.

The mitochondrial Ca2$ uptake was depressed by the calmodulin antagonists chlorpromazine hydrochloride (CPZ) and N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide hydrochloride (W-7). The Ca2$-ATPase from apple mitochondria was also inhibited by CPZ or W-7. The apparent Km value for Ca2$ in mitochondrial Ca2$ uptake (Km=0.35 mM) was similar to that of mitochondrial Ca2$-ATPase (Km=0.32 mM). The inhibitory effect of W-7 on the activity of the mitochondrial Ca2$ uptake was closely correlated with the inhibition by W-7 of mitochondrial Ca2$-ATPase (r=0.996). These findings indicate that the mitochondrial uptake of Ca2$ in apple fruit depends on the calmodulin-mediated activation of Ca2$-ATPase.

The microsomal Ca2$ uptake was depressed by CPZ, suggesting that the microsomal Ca2$ uptake may also be modulated by calmodulin.

1 Contribution No. C-72, Fruit Tree Research Station.

(Received June 7, 1982; Accepted October 19, 1982)
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