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Plant and Cell Physiology, 1982, Vol. 23, No. 8 1419-1425
© 1982

Article

Respiration-Dependent Membrane Hyperpolarization in Tonoplast-Free Cells of Nitella axilliformis*

Tetsuro Mimura, Teruo Shimmen and Masashi Tazawa

Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan

Cells of Nitella axilliformis were made tonoplast-free by intracellular perfusion of media containing ethyleneglycol-bis-(ß-aminoethylether)N,N'-tetraacetic acid (EGTA). When the perfusion medium contained ADP as well as ATP, the membrane hyperpolarized in darkness in a manner similar to light-induced hyperpolarization. This light-independent hyperpolarization seems to be due to activation of the electrogenic ion pump in the plasma membrane because the hyperpolarized value of the membrane potential was more negative than the equilibrium potential for K$, the most negative ion equilibrium potential in Nitella.

The hyperpolarization was inhibited by the respiratory chain inhibitors NaCN (1 mM), antimycin A (10 µM) and rotenone (10 µM). NaCN slightly decreased the ATP concentration in the cell perfused with medium containing 1 mM ATP and 1 mM ADP; but, even after treatment with NaCN, the cell had about 80% of the ATP value for the control.

* This study is dedicated to the late Professor J. Ashida.

(Received June 24, 1982; Accepted October 15, 1982)
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