Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Brune, D. C.
Right arrow Articles by González, I.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Brune, D. C.
Right arrow Articles by González, I.
Agricola
Right arrow Articles by Brune, D. C.
Right arrow Articles by González, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1982, Vol. 23, No. 8 1323-1328
© 1982

Article

Measurements of Photosynthetic Sulfide Oxidation by Chlorobium Using a Sulfide Ion Selective Electrode

Daniel C. Brune and Ileana González

Biology Department, University of Puerto Rico Rio Piedras, Puerto Rico 00931

A simple technique is described for using a sulfide sensitive electrode to measure the photooxidation of H2S by a green sulfur bacterium, Chlorobium limicola forma thiosulfatophilum. Sulfide photooxidation occurred only in the presence of bicarbonate at concentrations greater than 0.1 mM. This implies that the rate-limiting carboxylating enzyme for CO2 fixation in Chlorobium has a relatively low affinity for CO2 compared to ribulose-1,5-biphosphate carboxylase. Carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazone (FCCP), an uncoupler of photophosphorylation, delays sulfide oxidation for about 15 sec after the onset of illumination at 2 µM and is completely inhibitory at 10 µM. These effects can be explained by the ATP requirement for CO2 fixation. When the photooxidation of H2S was prevented by 10 µM FCCP, a photoevolution of H2S was observed.

(Received December 24, 1981; Accepted September 10, 1982)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
L. Lüthy, M. Fritz, and R. Bachofen
In Situ Determination of Sulfide Turnover Rates in a Meromictic Alpine Lake
Appl. Envir. Microbiol., February 1, 2000; 66(2): 712 - 717.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.