Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shioi, Y.
Right arrow Articles by Sasa, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Shioi, Y.
Right arrow Articles by Sasa, T.
Agricola
Right arrow Articles by Shioi, Y.
Right arrow Articles by Sasa, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1982, Vol. 23, No. 8 1315-1321
© 1982

Article

Separation of Protochlorophylls Esterified with Different Alcohols from Inner Seed Coats of Three Cucurbitaceae

Yuzo Shioi and Tsutomu Sasa

Division of Biology, Miyazaki Medical College Kiyotake, Miyazahi 889-16, Japan

Reversed-phase high-performance liquid chromatography was used to separate protochlorophyllide esters isolated from inner seed coats of three Cucurbitaceae. At least 17 protochlorophylls esterified with different alcohols were separated on an octadecyl silica (ODS) column from purified pigments of pumpkin (Cucurbita moschata) and balsam pear (Momordica charantica) seeds with a single elution using 100% methanol within 22 min. The separation was done according to the numbers of carbon atoms in the esterifying alcohol of the protochlorophyll molecule with high resolution and reproducibility. Gas chromatographic analysis of the pigment-esterifying alcohols confirmed the presence of a number of protochlorophylls esterified with different alcohols. This method permits rapid, efficient and quantitative detection and identification of chlorophylls in complex mixtures.

(Received May 17, 1982; Accepted September 6, 1982)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.