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Plant and Cell Physiology, 1982, Vol. 23, No. 7 1197-1203
© 1982

Article

Characterization of the mRNA for the {alpha}-Amylase from Wheat

Kiyoto Motojima1 and Kenji Sakaguchi

Laboratory of Microbiological Chemistry, Mitsubishi-Kasei Institute of Life Sciences Machida-shi, Tokyo 194, Japan

Characterization of the mRNA for the {alpha}-amylase from wheat was made by fractionation of the total membrane-bound polysomal RNA and by immunoprecipitation of in vitro synthesized {alpha}-amylase by its specific antibody. The content of the mRNA for the {alpha}-amylase in the membrane-bound polysomal fraction from germinating wheat seeds was estimated as 5–10% of the total mRNA in this fraction. RNA, separated in high resolution on acid-urea-agarose gels by electrophoresis, was recovered from the gels by a new method. Its translation products in rabbit reticulocyte lysates were analyzed with a specific antibody against {alpha}-amylase. The size of the mRNA for {alpha}-amylase was estimated as 5.0×105 daltons.

1 Present address: National Institutes of Health, Bldg. 10, Rm. 9B-15, Bethesda, MD 20205, U.S.A.

(Received March 6, 1982; Accepted August 4, 1982)
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