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Plant and Cell Physiology, 1982, Vol. 23, No. 5 929-933
© 1982

Short communication

Plasmid DNA-induced Transformability of Yeast Cells during Spheroplast Conversion1

Kazuo Yoshida and Keiko Takagi

Biological Institute, Faculty of Science, Nagoya University Nagoya 464, Japan

Changes in transformability, regenerability, cell fusibility and endocytotic activity of Saccharomyces cerevisiae yeast during spheroplast conversion were investigated using the cell wall digesting enzyme Zymolylase. The transformability with plasmid DNA, cell fusibility and endocytotic activity all reached maximum at the critical spheroplast conversion period when about half of the cells had been converted into spheroplasts. Lysosomotropic drugs, chloroquine and dansylcadaverine enhanced transformation and endocytosis two- to threefold. This study showed that a close relationship exists among transformability, cell fusing activity and endocytotic activity of spheroplasts.

1 A preliminary report appeared in ref. Yoshida and Takagi (1981).

(Received March 15, 1982; Accepted May 12, 1982)
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