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Plant and Cell Physiology, 1982, Vol. 23, No. 4 713-716
© 1982

Short communication

Embryogenesis and Plantlet Formation through Direct Culture of Isolated Pollen of Nicotiana tabacum cv. Samsum and Nicotiana rustica cv. Rustica

Jun Imamura1, Ekuko Okabe, Masaharu Kyo and Hiroshi Harada

Institute of Biological Sciences, The University of Tsukuba Sakura-mura, Ibaraki 305, Japan

Pollen embryos and plantlets of Nicotiana tabacum cv. Samsun and Nicotiana rustica cv. Rustica were obtained through direct pollen culture without prior treatment or prior culture of anthers or buds. Isolated pollen was cultured first in a medium without sucrose, then transferred into Nitsch's H medium containing 2% sucrose and 5 mM glutamine. The optimum medium for the initial culture was water and the optimum period of culture was ca. 6 days when binucleate pollen was used.

1 Present address: Friedrich Miescher Inst., P.O.B. 273, CH-4002 Basel, Switzerland.

(Received January 18, 1982; Accepted March 19, 1982)
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