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Plant and Cell Physiology, 1982, Vol. 23, No. 4 703-708
© 1982

Short communication

A High Performance Liquid Chromatography-Radio-Organic Acid Analyzer for Determining the Specific Activities of Radioactive Organic Acids

Satoshi Mori

Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan

To monitor rapidly the specific activity of radioactive organic acids, a high performance liquid chromatography (HPLC)-radioanalyzer was constructed. The instrument is a linear combination of an HPLC organic acid analyzer and a radioanalyzer. Organic acids are separated through a 100 cm column packed with Diaion-CK08S (H$type) resin. The organic acids were detected at 430 nm after reaction with bromocresol purple. The effluents from the HPLC are conduced to a U-type flow-cell packed with lithium-glass scintillator, which floresces in response to rrß-rays. The instrument was utilized to investigate the assimilation of H14CO3– fixation in barley roots after 10 min uptake. Malate showed the highest specific activity, followed by citrate and aspartate.

(Received August 17, 1981; Accepted March 17, 1982)
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