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Plant and Cell Physiology, 1982, Vol. 23, No. 4 657-662
© 1982

Article

Inhibition of Membrane-Bound Adenosine Triphosphatase Activity from Nicotiana tabacum L. Leaves with Alkyltrimethylammonium Bromide

Kunihiro Kasamo

Institute for Plant Virus Research Tsukuba Science City, Yatabe, Ibaraki 305, Japan

Cetyltrimethylammonium bromide (CTAB), a cationic detergent, more effectively inhibited the activity of membrane-bound epidermal adenosine triphosphatase (ATPase) of tobacco (Nicotiana tabacum L. cv. Samsun) leaves than anionic or non-ionic detergents. The inhibition of ATPase activity was highly dependent on the length of the alkyl chain of alkyltrimethylammonium: CTAB > dodecyltrimethylammonium bromide > n-octyltrimethylammonium bromide {fallingdotseq} trimethylammonium chloride {fallingdotseq} cetyl bromide, compared at 10–4 M. The last three derivatives hardly inhibited the activity. CTAB inhibition was equivalent to that due to other cationic detergents, cetylpyridinium bromide and cetyl amine, but less than that by gramicidin S and tyrocidine and stronger than that by N,N'-dicyclohexylcarbodiimide and vanadate.

These results show that a certain length of the alkyl chain (Cn>12) and the combination of both hydrophobic and charged groups of a detergent moiety are indispensable for inhibiting the membrane-bound epidermal ATPase activity.

(Received January 26, 1982; Accepted April 10, 1982)
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