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Plant and Cell Physiology, 1982, Vol. 23, No. 3 433-439
© 1982

Article

Comparison of Free and Bound Amino Acids in White and Green Tissues of Variegated Tobacco Plants

Yoko Tanabe and Nobumaro Kawashima

Central Research Institute, Japan Tobacco and Salt Public Corporation 6-2 Umegaoka. Yokohama, Kanagawa 227, Japan

Qualitative and quantitative comparisons of free and bound amino acids and soluble proteins in white and green tissues of variegated tobacco leaves were made. White tissue contained more free and less bound amino acids than green tissue, although the sum of the total amino acids did not differ significantly between the two tissues. The major free amino acids in white tissue were glutamine and asparagine, whereas those in green tissue were glutamic acid, aspartic acid and {gamma}-aminobutyric acid. The contents of fraction 1 protein and 70 S ribosomes in white tissue were negligible in comparison with those found in green tissue, but the amounts of other soluble protein components and the 80 S ribosomes were at the same level in both tissues.

(Received October 21, 1981; Accepted January 28, 1982)
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