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Plant and Cell Physiology, 1981, Vol. 22, No. 5 917-922
© 1981

Article

Photoautotrophic and Photomixotrophic Culture of Green Tobacco Cells in a Jar-Fermenter

Yasuyuki Yamada, Kazumitsu Imaizumi1, Fumihiko Sato and Takeshi Yasuda

Department of Agricultural Chemistry, Kyoto University Kyoto 606, Japan

Green tobacco cells were cultured photomixotrophically and photoautotrophically in a jar-fermenter (working volume, 5 liters). Optimum aeration and agitation, i.e. the type of impeller (marine), agitation speed (200 rpm), and aeration rate [1 aeration volume/medium volume/min (wm)], and light intensity (8,000 lux) were determined in order to get high cell growth. Under these conditions, tobacco cells increased about ten fold after 17 days of photomixotrophic culture. In the photoautotrophic culture that had aeration with 1% CO2 enriched air, the mass of the tobacco cells did not increase because stimulated cell respiration compensated for photosynthetic activity. A low oxygen supply was essential for photoautotrophic culture in a jar-fermenter. The fresh weight of green tobacco cells increased twice under photoautotrophy, after 17 days of culture with an agitation of 200 rpm, aeration of 0.8 vvm, with air containing 1% CO2, 14% O2 and 85% N2, and an illumination of 8,000 lux.

1Present address: Botanical Institute, PL-Women's College, Tondabayashi, Osaka 584, Japan.

(Received April 2, 1981; Accepted June 15, 1981)
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