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Plant and Cell Physiology, 1981, Vol. 22, No. 5 899-909
© 1981

Article

Studies on the Pectic Depolymerases I. Exopolygalacturonase from Root Tissues and Cell Suspension Cultures of Carrots

Haruyoshi Konno1, Kenji Katoh2 and Yoshiki Yamasaki1

1Institute for Agricultural and Biological Sciences, Okayama University Kurashiki, Okayama 710, Japan
2Suntory Institute for Bioorganic Research Shimamoto-cho, Mishima-gun, Osaka 618, Japan

Three polygalacturonases (PG) have been isolated from carrots (Daucus carota L. cv. Kintoki). Two were isolated from root tissues (PG-I and PG-II) and one from cell suspension cultures (PG-III). PG-I and PG-III were readily solubilized in a low ionic strength buffer, whereas PG-II required additional NaCl to be solubilized. These seems to be a change in the properties of PG between the original tissue and carrot cell cultures. The three PGs were partially purified by chromatography on Sephadex G-150, and characterized.

Elution from a Sephadex G-150 column indicated a molecular weight of about 48,000 for all three PGs. PG-III, studied in detail, hydrolyzed the galacturonan chain in an exo-fashion, and was not activated by a variety of cations at concentrations of 0.5 or 1.0 mM. The pH optimum, and pH and heat stability of PG-I and PG-III were slightly different from those of PG-II. PG-I was also different from PG-II and PG-III in its pectin hydrolyzing activity.

These results indicate that the enzymatic properties of PG-III from cell cultures are very similar to those of PG-I or PG-II from root tissues; the only significant difference seems to be the binding properties of the PGs to the cell wall materials.

(Received March 23, 1981; Accepted June 11, 1981)
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