Plant and Cell Physiology, 1981, Vol. 22, No. 5 867-880
© 1981
Article |
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
The Research Institute for Food.Science, Kyoto University Uji, Kyoto 611, Japan
Intact spinach chloroplasts scavenge hydrogen peroxide with a peroxidase that uses a photoreductant as the electron donor, but the activity of ruptured chloroplasts is very low [Nakano and Asada (1980) Plant & Cell Physiol. 21 : 1295]. Ruptured spinach chloroplasts recovered their ability to photoreduce hydrogen peroxide with the concomitant evolution of oxygen after the addition of glutathione and dehydroascorbate (DHA). In ruptured chloroplasts, DHA was photoreduced to ascorbate and oxygen was evolved in the process in the presence of glutathione. DHA reductase (EC 1.8.5.1
[EC]
) and a peroxidase whose electron donor is specific to L-ascorbate are localized in chloroplast stroma. These observations confirm that the electron donor for the scavenging of hydrogen peroxide in chloroplasts is L-ascorbate and that the L-ascorbate is regenerated from DHA by the system: photosystem I
ferredoxinNADPglutathione. A preliminary characterization of the chloroplast peroxidase is given.
(Received April 16, 1981; Accepted June 3, 1981)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. K. Tewari, P. Kumar, and P. N. Sharma Morphology and oxidative physiology of boron-deficient mulberry plants Tree Physiol, November 20, 2009; (2009) tpp093v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, S. Lu, X. Xu, H. Korpelainen, and C. Li Changes in antioxidant enzyme activities and isozyme profiles in leaves of male and female Populus cathayana infected with Melampsora larici-populina Tree Physiol, November 16, 2009; (2009) tpp094v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Matamoros, J. Loscos, K.-J. Dietz, P. M. Aparicio-Tejo, and M. Becana Function of antioxidant enzymes and metabolites during maturation of pea fruits J. Exp. Bot., October 11, 2009; (2009) erp285v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Im, M. Ji, A. Lee, R. Killens, A. M. Grunden, and W. F. Boss Expression of Pyrococcus furiosus Superoxide Reductase in Arabidopsis Enhances Heat Tolerance Plant Physiology, October 1, 2009; 151(2): 893 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yin, H. Sun, X. Xin, G. Qin, Z. Liang, and X. Jing Mitochondrial Damage in the Soybean Seed Axis During Imbibition at Chilling Temperatures Plant Cell Physiol., July 1, 2009; 50(7): 1305 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-J. Xia, Y.-J. Wang, Y.-H. Zhou, Y. Tao, W.-H. Mao, K. Shi, T. Asami, Z. Chen, and J.-Q. Yu Reactive Oxygen Species Are Involved in Brassinosteroid-Induced Stress Tolerance in Cucumber Plant Physiology, June 1, 2009; 150(2): 801 - 814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Muscolo and M. Sidari Carboxyl and Phenolic Humic Fractions Affect Pinus Nigra Callus Growth and Metabolism Soil Sci. Soc. Am. J., May 13, 2009; 73(4): 1119 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Shen, R. Krishnamurthy, and K.-W. Yeh Decreased L-Ascorbate Content Mediating Bolting is Mainly Regulated by the Galacturonate Pathway in Oncidium Plant Cell Physiol., May 1, 2009; 50(5): 935 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Delaplace, M.-L. Fauconnier, K. Sergeant, J.-F. Dierick, M. Oufir, F. van der Wal, A. H. P. America, J. Renaut, J.-F. Hausman, and P. du Jardin Potato (Solanum tuberosum L.) tuber ageing induces changes in the proteome and antioxidants associated with the sprouting pattern J. Exp. Bot., March 1, 2009; 60(4): 1273 - 1288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Lim, S. M. Kang, J. L. Cho, and K. C. Gross Antioxidizing Enzyme Activities in Chilling-sensitive and Chilling-tolerant Pepper Fruit as Affected by Stage of Ripeness and Storage Temperature J. Amer. Soc. Hort. Sci., January 1, 2009; 134(1): 156 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Costa e Silva, A. Shvaleva, F. Broetto, M.F. Ortuno, M.L. Rodrigues, M.H. Almeida, M.M. Chaves, and J.S. Pereira Acclimation to short-term low temperatures in two Eucalyptus globulus clones with contrasting drought resistance Tree Physiol, January 1, 2009; 29(1): 77 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hideg, P. B. Kos, and U. Schreiber Imaging of NPQ and ROS Formation in Tobacco Leaves: Heat Inactivation of the Water-Water Cycle Prevents Down-Regulation of PSII Plant Cell Physiol., December 1, 2008; 49(12): 1879 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rapala-Kozik, E. Kowalska, and K. Ostrowska Modulation of thiamine metabolism in Zea mays seedlings under conditions of abiotic stress J. Exp. Bot., November 1, 2008; 59(15): 4133 - 4143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loscos, M. A. Matamoros, and M. Becana Ascorbate and Homoglutathione Metabolism in Common Bean Nodules under Stress Conditions and during Natural Senescence Plant Physiology, March 1, 2008; 146(3): 1282 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pandey, S. Chakraborty, A. Datta, and N. Chakraborty Proteomics Approach to Identify Dehydration Responsive Nuclear Proteins from Chickpea (Cicer arietinum L.) Mol. Cell. Proteomics, January 1, 2008; 7(1): 88 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Hong, Y. T. Hsu, Y.-C. Tsai, and C. H. Kao Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl J. Exp. Bot., October 4, 2007; (2007) erm174v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Cervilla, B. Blasco, J. J. Rios, L. Romero, and J. M. Ruiz Oxidative Stress and Antioxidants in Tomato (Solanum lycopersicum) Plants Subjected to Boron Toxicity Ann. Bot., October 1, 2007; 100(4): 747 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Duan, Y. Yang, Y. Lu, H. Korpelainen, F. Berninger, and C. Li Interactions between water deficit, ABA, and provenances in Picea asperata J. Exp. Bot., August 1, 2007; 58(11): 3025 - 3036. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zederbauer, P. G. Furtmuller, M. Bellei, J. Stampler, C. Jakopitsch, G. Battistuzzi, N. Moguilevsky, and C. Obinger Disruption of the Aspartate to Heme Ester Linkage in Human Myeloperoxidase: IMPACT ON LIGAND BINDING, REDOX CHEMISTRY, AND INTERCONVERSION OF REDOX INTERMEDIATES J. Biol. Chem., June 8, 2007; 282(23): 17041 - 17052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Moradi and A. M. Ismail Responses of Photosynthesis, Chlorophyll Fluorescence and ROS-Scavenging Systems to Salt Stress During Seedling and Reproductive Stages in Rice Ann. Bot., June 1, 2007; 99(6): 1161 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wang and Y. Jiang Antioxidant Responses of Creeping Bentgrass Roots to Waterlogging Crop Sci., January 22, 2007; 47(1): 232 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. SINGH, D. R. BATISH, S. KAUR, K. ARORA, and R. K. KOHLI {alpha}-Pinene Inhibits Growth and Induces Oxidative Stress in Roots Ann. Bot., December 1, 2006; 98(6): 1261 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-T. Liu, Y.-Y. Liu, Q.-H. Pan, H.-R. Yang, J.-C. Zhan, and W.-D. Huang Novel interrelationship between salicylic acid, abscisic acid, and PIP2-specific phospholipase C in heat acclimation-induced thermotolerance in pea leaves J. Exp. Bot., September 1, 2006; 57(12): 3337 - 3347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Matamoros, J. Loscos, M. J. Coronado, J. Ramos, S. Sato, P. S. Testillano, S. Tabata, and M. Becana Biosynthesis of Ascorbic Acid in Legume Root Nodules Plant Physiology, July 1, 2006; 141(3): 1068 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pignocchi, G. Kiddle, I. Hernandez, S. J. Foster, A. Asensi, T. Taybi, J. Barnes, and C. H. Foyer Ascorbate Oxidase-Dependent Changes in the Redox State of the Apoplast Modulate Gene Transcript Accumulation Leading to Modified Hormone Signaling and Orchestration of Defense Processes in Tobacco Plant Physiology, June 1, 2006; 141(2): 423 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D'ARCY-LAMETA, R. FERRARI-ILIOU, D. CONTOUR-ANSEL, A.-T. PHAM-THI, and Y. ZUILY-FODIL Isolation and Characterization of Four Ascorbate Peroxidase cDNAs Responsive to Water Deficit in Cowpea Leaves Ann. Bot., January 1, 2006; 97(1): 133 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhou, Z. Guo, J. Xing, and B. Huang Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis J. Exp. Bot., December 1, 2005; 56(422): 3223 - 3228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-M. Li and H.-Y. Hu Isolation and Characterization of a Novel Antialgal Allelochemical from Phragmites communis Appl. Envir. Microbiol., November 1, 2005; 71(11): 6545 - 6553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-T. Shiu and T.-M. Lee Ultraviolet-B-induced oxidative stress and responses of the ascorbate-glutathione cycle in a marine macroalga Ulva fasciata J. Exp. Bot., November 1, 2005; 56(421): 2851 - 2865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-X. Yang, R.-Y. Wang, F. Ren, J. Liu, J. Cheng, and Y.-T. Lu AtGLB1 Enhances the Tolerance of Arabidopsis to Hydrogen Peroxide Stress Plant Cell Physiol., August 1, 2005; 46(8): 1309 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kuzniak and M. Sklodowska Compartment-specific role of the ascorbate-glutathione cycle in the response of tomato leaf cells to Botrytis cinerea infection J. Exp. Bot., March 1, 2005; 56(413): 921 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhou, Z. Guo, and Z. Liu Effects of Abscisic Acid on Antioxidant Systems of Stylosanthes guianensis (Aublet) Sw. under Chilling Stress Crop Sci., February 23, 2005; 45(2): 599 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-S. Chen and L. Cheng Both xanthophyll cycle-dependent thermal dissipation and the antioxidant system are up-regulated in grape (Vitis labrusca L. cv. Concord) leaves in response to N limitation J. Exp. Bot., September 1, 2003; 54(390): 2165 - 2175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Danna, C. G. Bartoli, F. Sacco, L. R. Ingala, G. E. Santa-Maria, J. J. Guiamet, and R. A. Ugalde Thylakoid-Bound Ascorbate Peroxidase Mutant Exhibits Impaired Electron Transport and Photosynthetic Activity Plant Physiology, August 1, 2003; 132(4): 2116 - 2125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pignocchi, J. M. Fletcher, J. E. Wilkinson, J. D. Barnes, and C. H. Foyer The Function of Ascorbate Oxidase in Tobacco Plant Physiology, July 1, 2003; 132(3): 1631 - 1641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dutilleul, M. Garmier, G. Noctor, C. Mathieu, P. Chetrit, C. H. Foyer, and R. de Paepe Leaf Mitochondria Modulate Whole Cell Redox Homeostasis, Set Antioxidant Capacity, and Determine Stress Resistance through Altered Signaling and Diurnal Regulation PLANT CELL, May 1, 2003; 15(5): 1212 - 1226. [Abstract] [Full Text]
|
|
|
|
|
|
M. Jiang and J. Zhang Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves J. Exp. Bot., December 1, 2002; 53(379): 2401 - 2410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Chiu, C. L. Chen, and J. M. Sung Effect of Priming Temperature on Storability of Primed sh-2 Sweet Corn Seed Crop Sci., November 1, 2002; 42(6): 1996 - 2003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. White and L. S. Jahnke Contrasting Effects of UV-A and UV-B on Photosynthesis and Photoprotection of {beta}-carotene in two Dunaliella spp. Plant Cell Physiol., August 15, 2002; 43(8): 877 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. I. Panchuk, R. A. Volkov, and F. Schoffl Heat Stress- and Heat Shock Transcription Factor-Dependent Expression and Activity of Ascorbate Peroxidase in Arabidopsis Plant Physiology, June 1, 2002; 129(2): 838 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. del Rio, F. J. Corpas, L. M. Sandalio, J. M. Palma, M. Gomez, and J. B. Barroso Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes J. Exp. Bot., May 15, 2002; 53(372): 1255 - 1272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shigeoka, T. Ishikawa, M. Tamoi, Y. Miyagawa, T. Takeda, Y. Yabuta, and K. Yoshimura Regulation and function of ascorbate peroxidase isoenzymes J. Exp. Bot., May 15, 2002; 53(372): 1305 - 1319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Payton, R. Webb, D. Kornyeyev, R. Allen, and A. S. Holaday Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity J. Exp. Bot., December 1, 2001; 52(365): 2345 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jiang and J. Zhang Effect of Abscisic Acid on Active Oxygen Species, Antioxidative Defence System and Oxidative Damage in Leaves of Maize Seedlings Plant Cell Physiol., November 1, 2001; 42(11): 1265 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bailly, C. Audigier, F. Ladonne, M. H. Wagner, F. Coste, F. Corbineau, and D. Come Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality J. Exp. Bot., April 15, 2001; 52(357): 701 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sano, M. Ueda, S. Kitajima, T. Takeda, S. Shigeoka, N. Kurano, S. Miyachi, C. Miyake, and A. Yokota Characterization of Ascorbate Peroxidases from Unicellular Red Alga Galdieria partita Plant Cell Physiol., April 1, 2001; 42(4): 433 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Venisse, G. Gullner, and M.-N. Brisset Evidence for the Involvement of an Oxidative Stress in the Initiation of Infection of Pear by Erwinia amylovora Plant Physiology, April 1, 2001; 125(4): 2164 - 2172. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Jiang and B. Huang Drought and Heat Stress Injury to Two Cool-Season Turfgrasses in Relation to Antioxidant Metabolism and Lipid Peroxidation Crop Sci., March 1, 2001; 41(2): 436 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang and B. Huang Effects of calcium on antioxidant activities and water relations associated with heat tolerance in two cool-season grasses J. Exp. Bot., February 1, 2001; 52(355): 341 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. P. Roxas, S. A. Lodhi, D. K. Garrett, J. R. Mahan, and R. D. Allen Stress Tolerance in Transgenic Tobacco Seedlings that Overexpress Glutathione S-Transferase/Glutathione Peroxidase Plant Cell Physiol., November 1, 2000; 41(11): 1229 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimaoka, A. Yokota, and C. Miyake Purification and Characterization of Chloroplast Dehydroascorbate Reductase from Spinach Leaves Plant Cell Physiol., October 1, 2000; 41(10): 1110 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Turcsanyi, T. Lyons, M. Plochl, and J. Barnes Does ascorbate in the mesophyll cell walls form the first line of defence against ozone? Testing the concept using broad bean (Vicia faba L.) J. Exp. Bot., May 1, 2000; 51(346): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. McKersie, J. Murnaghan, K. S. Jones, and S. R. Bowley Iron-Superoxide Dismutase Expression in Transgenic Alfalfa Increases Winter Survival without a Detectable Increase in Photosynthetic Oxidative Stress Tolerance Plant Physiology, April 1, 2000; 122(4): 1427 - 1438. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Matamoros, L. M. Baird, P. R. Escuredo, D. A. Dalton, F. R. Minchin, I. Iturbe-Ormaetxe, M. C. Rubio, J. F. Moran, A. J. Gordon, and M. Becana Stress-Induced Legume Root Nodule Senescence. Physiological, Biochemical, and Structural Alterations Plant Physiology, September 1, 1999; 121(1): 97 - 112. [Abstract] [Full Text]
|
|
|
|
|
|
A. Jiménez, J. A. Hernández, G. Pastori, L. A. del Río, and F. Sevilla Role of the Ascorbate-Glutathione Cycle of Mitochondria and Peroxisomes in the Senescence of Pea Leaves Plant Physiology, December 1, 1998; 118(4): 1327 - 1335. [Abstract] [Full Text]
|
|
|
|
|
|
A. González, K. L. Steffen, and J. P. Lynch Light and Excess Manganese . Implications for Oxidative Stress in Common Bean Plant Physiology, October 1, 1998; 118(2): 493 - 504. [Abstract] [Full Text]
|
|
|
|
|
|
S. Biemelt, U. Keetman, and G. Albrecht Re-Aeration following Hypoxia or Anoxia Leads to Activation of the Antioxidative Defense System in Roots of Wheat Seedlings Plant Physiology, February 1, 1998; 116(2): 651 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Mock, U. Keetman, E. Kruse, B. Rank, and B. Grimm Defense Responses to Tetrapyrrole-Induced Oxidative Stress in Transgenic Plants with Reduced Uroporphyrinogen Decarboxylase or Coproporphyrinogen Oxidase Activity Plant Physiology, January 1, 1998; 116(1): 107 - 116. [Abstract] [Full Text]
|
|
|
|
|
|
I. Iturbe-Ormaetxe, P. R. Escuredo, C. Arrese-Igor, and M. Becana Oxidative Damage in Pea Plants Exposed to Water Deficit or Paraquat Plant Physiology, January 1, 1998; 116(1): 173 - 181. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kvaratskhelia, S. J. George, and R. N. F. Thorneley Salicylic Acid Is a Reducing Substrate and Not an Effective Inhibitor of Ascorbate Peroxidase J. Biol. Chem., August 22, 1997; 272(34): 20998 - 21001. [Abstract] [Full Text] [PDF]
|
|