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Plant and Cell Physiology, 1981, Vol. 22, No. 5 807-818
© 1981

Article

Demonstration of Voltage Dependency of Light-induced Potential Change in Chara

Teruo Shimmen and Masashi Tazawa

Department of Botany, Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan

The electromotive force (Em) of the plasma membrane of the tonoplast-free cell of Chara australis decreased when the electrogenic pump was stopped by removing ATP or Mg2+ from the cell. Such a cell showed a rapid light-induced potential change (rLPC). Three factors were considered to be responsible for the generation of rLPC; removal of Mg-ATP, stoppage of the electrogenic pump and membrane depolarization per se.

Tonoplast-free cells having enough Mg-ATP occasionally showed small Em (–87— –116 mV) due to stoppage of the electrogenic pump. Since the rLPC was induced in such cells, removal of Mg-ATP cannot be the factor. Cells having large Em due to active pump activity also showed rLPC when the potential difference across the plasma membrane (Vm) was depolarized by an outward electric current; evidence that not the stoppage of the pump but membrane depolarization is a necessary condition for the generation of rLPC.

In the rLPC Vm always changed in the negative direction. However, calculation of Em revealed the existence of a reversal potential [ (Em)rev] toward which Em converged from either more negative or more positive values. The (Em)rev approximately coincided with the equilibrium potential for K+ across the plasma membrane. Intracellular anions occupying lower positions in the lyotropic series inhibited rLPC.

(Received February 9, 1981; Accepted May 16, 1981)
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