Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kato, G.
Right arrow Articles by Nakamura, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kato, G.
Right arrow Articles by Nakamura, M.
Agricola
Right arrow Articles by Kato, G.
Right arrow Articles by Nakamura, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1981, Vol. 22, No. 5 759-771
© 1981

Article

Involvement of Lectins in Rhizobium-Pea Recognition

Goro Kato, Yoshiharu Maruyama and Michinori Nakamura1

Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Hapten-inhibition studies showed that 3-O-methyl-D-glucose and methyl-{alpha}-D-mannopyranoside, which are strong sugar haptens of hemagglutination by pea seed lectins, inhibited (a) binding of pea seed lectins with Rhizobium leguminosarum J357 cells, (b) the precipitin reaction of pea seed lectins with a capsular polysaccharide from J357 cells and (c) adsorption of J357 cells to a pea root. When the capsular polysaccharide was absorb ed by the lectins or oxidized by periodate to remove the precipitin reactivity with the lectin, the inhibitory activity of the capsular polysaccharide towards the adsorption disappeared. I.ectins, which were isolated from the bathing solution of pea roots in acid buffer (pH 2.1), were similar to the seed lectins in sugar-binding specificity. The possible existence of lectins on pea root hair surface was shown by the indirect imraunofluorescent antibody technique in combination with the biotin-avidin system. These results suggest that host recognition in Rhizobium-pea. symbiosis is based on the interaction between rhizobial cells and host lectins.

1Present address: Nodai Research Institute, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156, Japan.

(Received February 18, 1981; Accepted May 2, 1981)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
P. van Rhijn, R. B. Goldberg, and A. M. Hirsch
Lotus corniculatus Nodulation Specificity Is Changed by the Presence of a Soybean Lectin Gene
PLANT CELL, August 1, 1998; 10(8): 1233 - 1250.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.