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Plant and Cell Physiology, 1981, Vol. 22, No. 5 737-746
© 1981

Article

Purification and Properties of the Diamine Oxidase from Vicia faba Leaves

Hitoshi Matsuda1 and Yonezo Suzuki2

1Science Education Institute of Osaka Prefecture Karita, Sumiyoshi-ku, Osaka 558, Japan
2Department of Biology, Faculty of Science, Osaka City University Sumiyoshi-ku, Osaka 558, Japan

Diamine oxidase (EC 1.4.3.6 [EC] ) from the leaves of Vicia faba was purified to homogeneity by polyacrylamide gel electrophoresis. The molecular weight estimated by Sephadex G-200 gel filtration was about 126,000. Sodium dodecyl sulfate gel electrophoresis gave a single band at the molecular weight of 74,000. The isoelectric point was at pH 7.2. The enzyme contained two copper atoms per mole of enzyme. Inhibition with phenylhydrazine showed that the Vicia enzyme contains one mole of the carbonyl group per mole of the enzyme. The amino acid composition of the enzyme also is described.

(Received February 23, 1981; Accepted April 7, 1981)
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