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Plant and Cell Physiology, 1980, Vol. 21, No. 6 979-988
© 1980

Article

Cytochrome b560 in chromatophores from Chromatium vinosum

Ken-ichiro Takamiya and Hiroyuki Hanada

Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka 812, Japan

A membrane-bound cytochrome of the B-type in Chromatium chromatophores, cytochrome b560, was reduced both by flash light activation and continuous illumination in the presence of antimycin at controlled ambient redox potentials. The light-minus-dark difference spectra had peaks at 560 and 430 nm, and troughs at 445 and 415 nm. The reduction was observed in the ambient redox potential from 400 to about 200 mV. However, below 200 mV, a re-reduction of photooxidized C-type cytochrome superimposed the reduction of cytochrome b560 In the absence of antimycin, the reduction was not observed, suggesting that the reoxidation of cytochrome b560 was faster than the reduction.

Dark titrations at various pH values showed that Em7 of the cytochrome b560 was about 40 mV and the Em value was pH-dependent (–60 mV/pH) from pH 6 to 9. Cytochrome b560 had a pK at around pH 9.

The content and some properties of cytochrome b560 were similar in chromatophores from either photoautotrophically or photoheterotrophically grown cells.

The possibility of involvement of cytochrome b560 in the photosynthetic electron transfer is discussed.

(Received April 19, 1980; )
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