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Plant and Cell Physiology, 1980, Vol. 21, No. 6 933-937
© 1980


Article

Osmotic potential of the epidermal cells of cucumber hypocotyls as affected by gibberellin and cotyledons

Masayuki Katsumi, Haruko Kazama and Noriko Kawamura1

Biology Department, International Christian University Osawa, Mitaka, Tokyo 181, Japan

By using light-grown cucumber (cv. Aonagajibae) seedlings with fully expanded cotyledons, the osmotic potential of the epidermal cells of the hypocotyl, as determined by the plasmolysis method was examined in relation to the effects of gibberellin (GA7) and cotyledons. GA7 at a dosage level of 0.05 µg/plant applied to the apical bud lowered the osmotic potential; the decrease was significant 5 hr after the GA7 treatment. Removal of the cotyledons, covering them with aluminum foil, or applying linuron, a DCMU analogue, to them caused an increase in the potential. In light-grown cucumber seedlings, the maintenance of a lower osmotic potential in the epidermal cells of the hypocotyl was concluded to be regulated by the photosynthetic activity of the cotyledon and also by gibberellin.

1Noriko Katsu, Kitano-Haitsu 203, Saginuma 1-9-10, Takatsu-ku, Kawasaki 218, Japan.


(Received May 30, 1980; )
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