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Plant and Cell Physiology, 1980, Vol. 21, No. 6 1095-1107
© 1980

Article

Mineral nutrition, of cultured chlorophyllous cells of tobacco VII. Effects of the NH4/NO3 ratio and of Cl in the media on the growth and ionic balance of cells

Fumitake Yoshida, Setsuko Shimizu and Hitoshi Kohno

Laboratory of Soils and Fertilizers, Faculty of Agriculture, Tamagawa University Machida-shi, Tokyo 194, Japan

NG, a strain of cultured tobacco cells of Nicotiana glutinosa had high growth rates and carboxylate contents (C—A) of 100 to 130 meq/100 g of dry cells on media containing 42 meq NO3/liter as the sole N source. (C—A) is the amount of inorganic cations minus inorganic anions in meq per 100 g of dry cells.

NG, cultured on media containing NH4 10+NO3 42 in meq/liter, had lower growth rates and lower (C—A) values as compared with NG on media containing NO3 as the sole N source.

NG, cultured on media containing NH4 30+NO3 42 in meq/liter, had high growth rates and (A—C) values of 22 to 53 meq/100g of dry cells. In this case, the (A—C) content may correspond to organic cations, basic organic N compounds such as free as protein-bound basic amino acids. The easily absorbed Cl may have been required maintain good growth conditions such as ionic balance and a favorable pH in the cells.

Thus cultured cells of Nicotiana glutinosa may have physiological adaptability against variations in a relatively wide range of |C—A| contents [|C—A| being the absolute values of (C—A)].

(Received May 15, 1980; )
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