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Plant and Cell Physiology, 1980, Vol. 21, No. 6 1041-1051
© 1980

Article

Induction, selection and isolation of auxin heterotrophic and auxin-resistant mutants from cultured crown gall cells irradiated with gamma rays

Shigeaki Atsumi

Department of Biology, College of General Education, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan

Cultured crown gall cells were irradiated with gamma rays to induce mutation in indoleacetic acid biosynthesis. The irradiated cells were plated on a selection medium which contained auxin. Mutant cells adapted to selection media were characterized as auxin-heterotrophic and auxin-resistant cell lines. The auxin-heterotrophic mutants contained little auxin, whereas the auxin-resistant and -autotrophic mutants contained large amounts of auxin even when cultured with 0.3 ppm of 2,4-dichlorophenoxyacetic acid. Each mutant cell line contained as much octopine as its parental cells. The mutation rate was calculated as in the order of 10–8.

(Received May 6, 1980; )
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